Nicollette Gino led the Lancers as she has the past three rounds, shooting an 84 to sit alone in 10th place individually.
CBU posted a 361 to stay five shots ahead of Indiana-Purdue Fort Wayne in the fifth spot.
Kirsten Keyser sits a stroke behind Gino after shooting an 85 to put her in a tie for 11th. There were just three scores in the 70's during the first day, none lower than a 77.
Tournament host Butler leads the field with a 323 and Alabama State sits 10 strokes back in second place.
The Lancers conclude their two-tournament Florida swing tomorrow with the final round of the Bulldog Invitational.